Myocardin regulates bmp10 expression and is required for heart development. Hek293t cells were transfected for bmp10 lentiviral expression and egfp control virus production and purification according to the manufacturers instructions cell biolabs. Empty backbone a bicistronic lentiviral mammalian expression vector with a multiple cloning site and gfp. Lentiviral bicistronic expression vector iresbsd cell biolabs, inc.
Irescontaining bicistronic vectors allow the simultaneous expression of two proteins separately, but from the same rna transcript jackson et al. Lentiviral vector systems for constitutive gene expression. The vector contains an internal ribosomal entry site ires which allows a geneofinterest and the tdtomato fluorescent protein to be simultaneously coexpressed from a single mrna transcript. Aavdj helper free bicistronic expression system gfp. The ires vectors included with the bicistronic lentiviral expression systems each contain a multiple cloning site located upstream of the ires from which a selection marker is expressed. Clone your gene of interest and a genespecific promoter into one of this lentiviral expression vector, then cotransfect along with lentiviral packaging vectors into a packaging cell line such as 293t or 293ltv. Correlation of iresgfp fluorescence with expression level of 5.
Introduction the virapower lentiviral expression system allows creation of a replication incompetent, hiv1based lentivirus that is used to deliver and express your gene of interest in either dividing or nondividing mammalian cells. Clone your gene of interest into this lentiviral expression vector, then cotransfect along with lentiviral packaging vectors into a packaging cell line such as 293ltv. Oct 01, 2012 the mouse bmp10 cdna was generated via pcr and subcloned into the psmpuwiresgfp lentiviral expression vector. Product data sheet psmpuwiresgfp lentiviral expression vector. Is it possible that the ires element in this vector stops the ribosome coming from. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our virasafe packaging vectors to produce the highest viral titer.
This makes the system truly universal by allowing you to introduce your own promoter. Clone your gene of interest into this lentiviral expression vector, then co transfect along with lentiviral. Lentiviral bicistronic expression vector iresneo cell biolabs, inc. Lentivirus vector based on the human immunodeficiency virus1. Empty backbone lentiviral empty cloning vector containing ires and gfp. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to.
Expression system is designed to minimize the chance of generating replicationcompetent lentivirus, but precautions should still be taken to avoid direct contact with viral supernatants. Cellbased assays 5 stem cell research 31 viral expression 39. Use text editor or plasmid mapping software to view sequence. Pdf myocardin regulates bmp10 expression and is required. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our virasafe packaging vectors to. Because your gene of interest and a selection marker are. The mouse bmp10 cdna was generated via pcr and subcloned into the psmpuwiresgfp lentiviral expression vector. Lentiviral expression vector puro cell biolabs, inc.